KMID : 0624620110440060375
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BMB Reports 2011 Volume.44 No. 6 p.375 ~ p.380
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Carboxy-terminus truncations of Bacillus licheniformis SK-1 CHI72 with distinct substrate specificity
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Kudan Sanya
Kuttiyawong Kamontip Pichyangkura Rath
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Abstract
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Bacillus licheniformis SK-1 naturally produces chitinase 72 (CHI72) with two truncation derivatives at the C-terminus, one with deletion of the chitin binding domain (ChBD), and the other with deletions of both fibronectin type III domain (FnIIID) and ChBD. We constructed deletions mutants of CHI72 with deletion of ChBD (CHI72¥ÄChBD) and deletions of both FnIIID and ChBD (CHI72¥ÄFnIIID¥ÄChBD), and studied their activity on soluble, amorphous and crystalline substrates. Interestingly, when equivalent amount of specific activity of each enzyme on soluble substrate was used, the product yield from CHI72- ¥ÄChBD and CHI72¥ÄFnIIID¥ÄChBD on colloidal chitin was 2.5 and 1.6 fold higher than CHI72, respectively. In contrast, the product yield from CHI72¥ÄChBD and CHI72¥ÄFnIIID- ¥ÄChBD on ¥Â-chitin reduced to 0.7 and 0.5 fold of CHI72, respectively. These results suggest that CHI72 can modulate its substrate specificities through truncations of the functional domains at the C-terminus, producing a mixture of enzymes with elevated efficiency of hydrolysis.
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KEYWORD
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Bacillus licheniformis SK-1, Chitin binding domain, C-terminus, Fibronectin type III domain, Substrate specificity
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